Rhinoz8142
Well-Known Member
Could someone assess me on this question...
Evaluate the contribution of Pasteur and Koch to our understanding
of infectious disease
Pasteur's contribution towards the understanding of infectious disease was that he disproved the theory of spontaneous generation.
Which people believed before Pasteur's contribution that they came into existence directly from non-living matter
- He hypothesised that microbes were in the air everywhere, and food spoils when these microbes land there and become active.
Pasteur poured broth into 2 flask one swan-necked flasks and another without a swan-neck top , and boiled both of them to kill off all microbes.
The flask with the broth open to the air developed cloudy bacterial growths, while the flask with the swan-neck stayed clear.
Which showed that micro-organisms were not spontaneously generated, if it was then both flask should have contained micro organisms.
Koch provide further proof that microscopic pathogens cause disease.
He obtained infected substance from a sheep suffering from anthrax
He placed it on a slide, observed it under a microscope and saw active rod-shaped cells
He established that the blood of animals with the disease always contained these micro-organisms, while the blood of healthy animals did not.
He found that if blood from an infected animal was injected into a healthy animal, it would cause disease.
He grew cultures of the rod-shaped bacteria to infect mice; they developed the disease. This proved that it was the bacteria, and not any other blood component that caused disease.
From this experiment he constructed his own method of establishing a certain microbe causes a disease called Koch Postulates
Which is
Step 1: All infected hosts must contain the suspect organism.
Step 2: A pure culture of the suspect organism must be obtained.
Step 3: A healthy organism infected with the pure culture must have the same symptoms as the original host.
Step 4: The suspect organism must be isolated from the second host, grown in pure culture and prove to be identical to the first culture
Evaluate the contribution of Pasteur and Koch to our understanding
of infectious disease
Pasteur's contribution towards the understanding of infectious disease was that he disproved the theory of spontaneous generation.
Which people believed before Pasteur's contribution that they came into existence directly from non-living matter
- He hypothesised that microbes were in the air everywhere, and food spoils when these microbes land there and become active.
Pasteur poured broth into 2 flask one swan-necked flasks and another without a swan-neck top , and boiled both of them to kill off all microbes.
The flask with the broth open to the air developed cloudy bacterial growths, while the flask with the swan-neck stayed clear.
Which showed that micro-organisms were not spontaneously generated, if it was then both flask should have contained micro organisms.
Koch provide further proof that microscopic pathogens cause disease.
He obtained infected substance from a sheep suffering from anthrax
He placed it on a slide, observed it under a microscope and saw active rod-shaped cells
He established that the blood of animals with the disease always contained these micro-organisms, while the blood of healthy animals did not.
He found that if blood from an infected animal was injected into a healthy animal, it would cause disease.
He grew cultures of the rod-shaped bacteria to infect mice; they developed the disease. This proved that it was the bacteria, and not any other blood component that caused disease.
From this experiment he constructed his own method of establishing a certain microbe causes a disease called Koch Postulates
Which is
Step 1: All infected hosts must contain the suspect organism.
Step 2: A pure culture of the suspect organism must be obtained.
Step 3: A healthy organism infected with the pure culture must have the same symptoms as the original host.
Step 4: The suspect organism must be isolated from the second host, grown in pure culture and prove to be identical to the first culture
