Modelling Pasteur (1 Viewer)

[Xtiiin3]

Hi, anyone have ideas on how to model Pasteur's experiment, or done something similar? Thanks!

 

[zingerburger]

You would have done this in class. Well, at least you should have.

View attachment 16449

What you need:

• Two conical flasks each with about 100mL of broth, chicken stock or whatever
• Two rubber stoppers - one should have a straight glass tube and the other should have a "gooseneck" tube (see attached images)
• Bunsen with tripod and gauze
• Love and care

What to do:

1. Boil the broth with the stoppers plugged in to kill any bacteria. With the gooseneck, make sure you don't boil too strongly, otherwise the liquid will "spit" and moisture will get into the lower bend.
2. Place the flasks in a dark place like a cupboard and leave it for a couple of weeks.
3. This is where love and care come in. Sing to the broth. Lovingly cradle them. Do whatever it takes to give the bacteria the maximum chance to fester and grow into a demonous horde.
4. Conquer Minas Tirith with your army of bacteria (optional).

Does that help?

 

[fretsonfire74]

You would have done this in class. Well, at least you should have.

View attachment 16449

What you need:

• Two conical flasks each with about 100mL of broth, chicken stock or whatever
• Two rubber stoppers - one should have a straight glass tube and the other should have a "gooseneck" tube (see attached images)
• Bunsen with tripod and gauze
• Love and care

What to do:

1. Boil the broth with the stoppers plugged in to kill any bacteria. With the gooseneck, make sure you don't boil too strongly, otherwise the liquid will "spit" and moisture will get into the lower bend.
2. Place the flasks in a dark place like a cupboard and leave it for a couple of weeks.
3. This is where love and care come in. Sing to the broth. Lovingly cradle them. Do whatever it takes to give the bacteria the maximum chance to fester and grow into a demonous horde.
4. Conquer Minas Tirith with your army of bacteria (optional).

Does that help?

This is pretty much it.


You have an open flask and one with a swan neck.
Put a nutrient rich broth in each (smells alot like vegemite) and boil. You leave the open one open for about a week. Basically record bacteria/fungal growths and describe colour/shape etc.
Never take the swan neck out because then the micro-organisms will have access to it.

 

[Mythology]

Procedure:

1. Use a meat extract cube to make a clear broth.
2. Use two conical flasks instead of Pasteur's balloon flasks. Fit the flasks with one-holed stoppers. Use glass tubing bent into an S-shape to replace Pasteur's swan-necked flask. Place a straight piece of glass tubing in the other flask.
3. Put some broth into both flasks and boil gently for fifteen minutes.
4. Leave both flasks, not in direct sunlight for several weeks. Every two or three days compare the contents of the two flasks. Look for cloudiness, scum, bubbles and mould colonies. Record your results.

*You can usually find eperiments on the HSC Online site!
This site has quick summaries of all the syllabus dot points!!

 

[*FROOGIE*]

Aren't You forgetting about using a control for the experiment????
You need to have a flask that has not been exposed to the air therefore will grow microbes etc this will allow you to compare the amount of microbes it any with the swan/goose neck and the straight neck. If you want to increase validilty and realibilty of your experiment you need to have a control. Also it is also a good idea to have one flask fully exposed to air. Goodluck